Beta 1


Title Cancer diagnostic : An automated method for determining HER2/neu status in breast cancer
Author Berg, Niels Teilmann
Supervisor Ersbøll, Bjarne Kjær (Image Analysis and Computer Graphics, Department of Informatics and Mathematical Modeling, Technical University of Denmark, DTU, DK-2800 Kgs. Lyngby, Denmark)
Institution Technical University of Denmark, DTU, DK-2800 Kgs. Lyngby, Denmark
Thesis level Master's thesis
Year 2008
Abstract Worldwide breast cancer is the fifth most common cause of cancer death and is the leading type of cancer in women. Breast cancer is predominantly a western problem but as western lifestyle is spreading the number of breast cancer cases rises worldwide. About 20% of all breast cancer cases have an amplification of the HER2/neu gene. This gene contributes to the growth and division of the cells and exists normally in healthy cells. When the gene is amplified by cancer both the growth and cell division happens faster making the cancer spread more aggressively and faster. Determination of the HER2/neu status is therefore important both for making a prognosis and as a target for therapy. In this thesis a method for automatically determining the HER2/neu status using imaging analysis is proposed and tested. The input images are captured from immunohistochemistry stained tumor biopsies. The staining colors cell membranes brown if the cells have an amplification of HER2/neu. The intensity of these brown membranes will be used as quantitative measure for the HER2/neu status. Four factors that influence the images or the staining and their effect on the brown intensity will be analyzed. These factors are: The two most common staining reagents, Dako’s HercepTest and Ventana’s Pathway®; images captured with two different cameras, the PixeLINK A686 and the Olympus DP 71 camera; the effect of the slice thickness and the effect of un-focused images. It is proven that the brown intensity, found by the method, can be used as measure for the HER2/neu status and that the effects of the factors either are inconsequential or that it is possible to modify the process to encompass them.
Series IMM-M.Sc.-2008-14
Fulltext
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Admin Creation date: 2008-02-19    Update date: 2010-10-28    Source: dtu    ID: 211033    Original MXD